2yif

Crystal structure of a F. nucleatum FMN riboswitch - Free state

spinqualitylabelsall models 2yif, resolution 3.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2yif is a 2 chain structure with sequence from Fusobacterium nucleatum subsp. nucleatum ATCC 25586. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.298Å Ligands: GTP, K, MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Understanding the nature of the free state of riboswitch aptamers is important for illuminating common themes in gene regulation by riboswitches. Prior evidence indicated the flavin mononucleotide (FMN)-binding riboswitch aptamer adopted a 'bound-like' structure in absence of FMN, suggesting only local conformational changes upon ligand binding. In the scope of pinpointing the general nature of such changes at the nucleotide level, we performed SHAPE mapping experiments using the aptamer domain of two phylogenetic variants, both in absence and in presence of FMN. We also solved the crystal structures of one of these domains both free (3.3 A resolution) and bound to FMN (2.95 A resolution). Our comparative study reveals that structural rearrangements occurring upon binding are restricted to a few of the joining regions that form the binding pocket in both RNAs. This type of binding event with minimal structural perturbations is reminiscent of binding events by conformational selection encountered in other riboswitches and various RNAs. Molecular sensing by the aptamer domain of the FMN riboswitch: a general model for ligand binding by conformational selection.,Vicens Q, Mondragon E, Batey RT Nucleic Acids Res. 2011 Oct 1;39(19):8586-98. Epub 2011 Jul 10. PMID:21745821 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Riboswitch 3D structures

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