3ahp

Crystal structure of stable protein, CutA1, from a psychrotrophic bacterium Shewanella sp. SIB1

spinqualitylabelsall models 3ahp, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3ahp is a 6 chain structure with sequence from Shewanella sp. SIB1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.7Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function E7FH74_9GAMM Publication Abstract from PubMed CutA1 is widely found in bacteria, plants and animals, including humans. The functions of CutA1, however, have not been well clarified. It is known that CutA1s from Pyrococcus horikoshii, Thermus thermophilus and Oryza sativa unfold at temperatures remarkably higher than the growth temperatures of the host organisms. In this work the crystal structure of CutA1 from the psychrotrophic bacterium Shewanella sp. SIB1 (SIB1-CutA1) in a trimeric form was determined at 2.7 A resolution. This is the first crystal structure of a psychrotrophic CutA1. The overall structure of SIB1-CutA1 is similar to those of CutA1 from Homo sapiens, Escherichia coli, Pyrococcus horikoshii, Thermus thermophilus, Termotoga maritima, Oryza sativa and Rattus norvergicus. A peculiarity is observed in the beta2 strand. The beta2 strand is divided into two short beta strands, beta2a and beta2b, in SIB1-CutA1. A thermal denaturation experiment revealed that SIB1-CutA1 does not unfold completely at 363 K at pH 7.0, although Shewanella sp. SIB1 cannot grow at temperatures exceeding 303 K. These results indicate that the trimeric structural motif of CutA1 is the critical factor in its unusually high stability and suggest that CutA1 needs to maintain its high stability in order to function, even in psychrotrophs. Crystal structure of stable protein CutA1 from psychrotrophic bacterium Shewanella sp. SIB1.,Sato A, Yokotani S, Tadokoro T, Tanaka SI, Angkawidjaja C, Koga Y, Takano K, Kanaya S J Synchrotron Radiat. 2011 Jan;18(1):6-10. Epub 2010 Nov 12. PMID:21169681 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also CutA1 3D structures

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