3awp

Cytochrome P450SP alpha (CYP152B1) mutant F288G

spinqualitylabelsall models 3awp, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3awp is a 1 chain structure with sequence from Sphingomonas paucimobilis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.8Å Ligands: HEM, MRD, PLM Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function O24782_SPHPI Publication Abstract from PubMed Cytochrome P450(SPalpha) (CYP152B1) isolated from Sphingomonas paucimobilis is the first P450 to be classified as a H(2)O(2)-dependent P450. P450(SPalpha) hydroxylates fatty acids with high alpha-regioselectivity. Herein we report the crystal structure of P450(SPalpha) with palmitic acid as a substrate at a resolution of 1.65 A. The structure revealed that the C(alpha) of the bound palmitic acid in one of the alternative conformations is 4.5 A from the heme iron. This conformation explains the highly selective alpha-hydroxylation of fatty acid observed in P450(SPalpha). Mutations at the active site and the F-G loop of P450(SPalpha) did not impair its regioselectivity. The crystal structures of mutants (L78F and F288G) revealed that the location of the bound palmitic acid was essentially the same as that in the WT, although amino acids at the active site were replaced with the corresponding amino acids of cytochrome P450(BSbeta) (CYP152A1), which shows beta-regioselectivity. This implies that the high regioselectivity of P450(SPalpha) is caused by the orientation of the hydrophobic channel, which is more perpendicular to the heme plane than that of P450(BSbeta). Crystal structure of H2O2-dependent cytochrome P450SPalpha with its bound fatty acid substrate: insight into the regioselective hydroxylation of fatty acids at the alpha position.,Fujishiro T, Shoji O, Nagano S, Sugimoto H, Shiro Y, Watanabe Y J Biol Chem. 2011 Aug 26;286(34):29941-50. Epub 2011 Jun 30. PMID:21719702 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Cytochrome P450 3D structures

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