3c1t

Binding of two substrate analogue molecules to dihydroflavonol 4-reductase alters the functional geometry of the catalytic site

spinqualitylabelsall models 3c1t, resolution 2.25Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3c1t is a 4 chain structure with sequence from Vitis vinifera. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.252Å Ligands: MYC, NAP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function DFRA_VITVI Bifunctional enzyme involved in flavonoid metabolism.[UniProtKB:Q9XES5] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Dihydroflavonol 4-reductase (DFR) is a key enzyme of the flavonoid biosynthesis pathway which catalyses the NADPH-dependent reduction of 2R,3R-trans-dihydroflavonols to leucoanthocyanidins. The latter are the precursors of anthocyans and condensed tannins, two major classes of phenolic compounds that strongly influence the organoleptic properties of wine. DFR has been investigated in many plant species, but little was known about its structural properties until the three-dimensional structure of the Vitis vinifera enzyme complexed with NADP(+) and its natural substrate dihydroquercetin (DHQ) was described. In the course of the study of substrate specificity, crystals of DFR-NADP(+)-flavonol (myricetin and quercetin) complexes were obtained. Their structures exhibit major changes with respect to that of the abortive DFR-NADP(+)-DHQ complex. Two flavonol molecules bind to the catalytic site in a stacking arrangement and alter its geometry, which becomes incompatible with enzymatic activity. The X-ray structures of both DFR-NADP(+)-myricetin and DFR-NADP(+)-quercetin are reported together with preliminary spectroscopic data. The results suggest that flavonols could be inhibitors of the activity of DFR towards dihydroflavonols. Structural evidence for the inhibition of grape dihydroflavonol 4-reductase by flavonols.,Trabelsi N, Petit P, Manigand C, Langlois d'Estaintot B, Granier T, Chaudiere J, Gallois B Acta Crystallogr D Biol Crystallogr. 2008 Aug;D64(Pt 8):883-91. Epub 2008, Jul 17. PMID:18645237 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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