3enu

Crystal structure of Nitrollin, a betagamma-crystallin from Nitrosospira multiformis

spinqualitylabelsall models 3enu, resolution 1.86Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3enu is a 1 chain structure with sequence from Nitrosospira multiformis ATCC 25196. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.86Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q2YAE2_NITMU Publication Abstract from PubMed The betagamma-crystallin superfamily has a well-characterized protein fold, with several members found in both prokaryotic and eukaryotic worlds. A majority of them contain two betagamma-crystallin domains. A few examples, such as ciona crystallin and spherulin 3a exist that represent the eukaryotic single-domain proteins of this superfamily. This study reports the high-resolution crystal structure of a single-domain betagamma-crystallin protein, nitrollin, from the ammonium-oxidizing soil bacterium Nitrosospira multiformis. The structure retains the characteristic betagamma-crystallin fold despite a very low sequence identity. The protein exhibits a unique case of homodimerization in betagamma-crystallins by employing its N-terminal extension to undergo three-dimensional (3D) domain swapping with its partner. Removal of the swapped strand results in partial loss of structure and stability but not dimerization per se as determined using gel filtration and equilibrium unfolding studies. Overall, nitrollin represents a distinct single-domain prokaryotic member that has evolved a specialized mode of dimerization hitherto unknown in the realm of betagamma-crystallins. Three-dimensional domain swapping in nitrollin, a single-domain betagamma-crystallin from Nitrosospira multiformis, controls protein conformation and stability but not dimerization.,Aravind P, Suman SK, Mishra A, Sharma Y, Sankaranarayanan R J Mol Biol. 2009 Jan 9;385(1):163-77. Epub 2008 Oct 19. PMID:18976659 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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