3h4s is a 2 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
KN14E_ARATH Minus-end microtubule-dependent motor protein involved in the regulation of cell division and trichome morphogenesis through microtubules bundling. Possesses basal and microtubule-stimulated ATPase activities. Acts as a hub that brings together microtubules and actin filaments to modulate the cytoskeleton during trichome formation and morphogenesis (PubMed:26287478). Could be involved in the negative regulation of root growth (PubMed:25262228).[1][2][3][4][5][6][7][8][9][10][11]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Much of the transport, tension, and movement in mitosis depends on kinesins, the ATP-powered microtubule-based motors. We report the crystal structure of a kinesin complex, the mitotic kinesin KCBP bound to its principal regulator KIC. Shown to be a Ca(2+) sensor, KIC works as an allosteric trap. Extensive intermolecular interactions with KIC stabilize kinesin in its ADP-bound conformation. A critical component of the kinesin motile mechanism, called the neck mimic, switches its association from kinesin to KIC, stalling the motor. KIC denies access of the motor to its track by steric interference. Two major features of this regulation, allosteric trapping and steric blocking, are likely to be general for all kinesins.
Structure of the complex of a mitotic kinesin with its calcium binding regulator.,Vinogradova MV, Malanina GG, Reddy AS, Fletterick RJ Proc Natl Acad Sci U S A. 2009 May 19;106(20):8175-9. Epub 2009 May 5. PMID:19416847[12]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑Kao YL, Deavours BE, Phelps KK, Walker RA, Reddy AS. Bundling of microtubules by motor and tail domains of a kinesin-like calmodulin-binding protein from Arabidopsis: regulation by Ca(2+)/Calmodulin. Biochem Biophys Res Commun. 2000 Jan 7;267(1):201-7. doi: 10.1006/bbrc.1999.1896. PMID:10623599 doi:https://dx.doi.org/10.1006/bbrc.1999.1896
↑Reddy VS, Day IS, Thomas T, Reddy AS. KIC, a novel Ca2+ binding protein with one EF-hand motif, interacts with a microtubule motor protein and regulates trichome morphogenesis. Plant Cell. 2004 Jan;16(1):185-200. Epub 2003 Dec 19. PMID:14688294 doi:10.1105/tpc.016600
↑Reddy AS. Analysis of calcium/calmodulin regulation of a plant kinesin using co-sedimentation and ATPase assays. Methods Mol Biol. 2007;392:23-36. doi: 10.1007/978-1-59745-490-2_2. PMID:17951708 doi:https://dx.doi.org/10.1007/978-1-59745-490-2_2
↑Humphrey TV, Haasen KE, Aldea-Brydges MG, Sun H, Zayed Y, Indriolo E, Goring DR. PERK-KIPK-KCBP signalling negatively regulates root growth in Arabidopsis thaliana. J Exp Bot. 2015 Jan;66(1):71-83. doi: 10.1093/jxb/eru390. Epub 2014 Sep 26. PMID:25262228 doi:https://dx.doi.org/10.1093/jxb/eru390
↑Tian J, Han L, Feng Z, Wang G, Liu W, Ma Y, Yu Y, Kong Z. Orchestration of microtubules and the actin cytoskeleton in trichome cell shape determination by a plant-unique kinesin. Elife. 2015 Aug 19;4. doi: 10.7554/eLife.09351. PMID:26287478 doi:https://dx.doi.org/10.7554/eLife.09351
↑Reddy AS, Safadi F, Narasimhulu SB, Golovkin M, Hu X. A novel plant calmodulin-binding protein with a kinesin heavy chain motor domain. J Biol Chem. 1996 Mar 22;271(12):7052-60. PMID:8636137
↑Song H, Golovkin M, Reddy AS, Endow SA. In vitro motility of AtKCBP, a calmodulin-binding kinesin protein of Arabidopsis. Proc Natl Acad Sci U S A. 1997 Jan 7;94(1):322-7. doi: 10.1073/pnas.94.1.322. PMID:8990207 doi:https://dx.doi.org/10.1073/pnas.94.1.322
↑Oppenheimer DG, Pollock MA, Vacik J, Szymanski DB, Ericson B, Feldmann K, Marks MD. Essential role of a kinesin-like protein in Arabidopsis trichome morphogenesis. Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6261-6. PMID:9177205
↑Narasimhulu SB, Kao YL, Reddy AS. Interaction of Arabidopsis kinesin-like calmodulin-binding protein with tubulin subunits: modulation by Ca(2+)-calmodulin. Plant J. 1997 Nov;12(5):1139-49. doi: 10.1046/j.1365-313x.1997.12051139.x. PMID:9418053 doi:https://dx.doi.org/10.1046/j.1365-313x.1997.12051139.x
↑Narasimhulu SB, Reddy AS. Characterization of microtubule binding domains in the Arabidopsis kinesin-like calmodulin binding protein. Plant Cell. 1998 Jun;10(6):957-65. PMID:9634584
↑Vinogradova MV, Malanina GG, Reddy AS, Fletterick RJ. Structure of the complex of a mitotic kinesin with its calcium binding regulator. Proc Natl Acad Sci U S A. 2009 May 19;106(20):8175-9. Epub 2009 May 5. PMID:19416847
