3h8i

The first X-ray structure of a sulfide:quinone oxidoreductase: Insights into sulfide oxidation mechanism

spinqualitylabelsall models 3h8i, resolution 2.65Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3h8i is a 2 chain structure with sequence from Acidianus ambivalens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.65Å Ligands: FAD, PO4, S3H Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function SQRD_ACIAM Catalyzes the oxidation of sulfides, such as hydrogen sulfide, with the help of a quinone. Has the highest activity with caldariella quinone and decylubiquinone, and lower activity with naphtoquinones. Consecutive reaction cycles lead to the accumulation of a polysulfide product on the active site Cys residues; these products are released when they exceed a critical length, typically as cyclooctasulfur. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed A sulfide:quinone oxidoreductase (SQR) was isolated from the membranes of the hyperthermoacidophilic archaeon Acidianus ambivalens, and its X-ray structure, the first reported for an SQR, was determined to 2.6 A resolution. This enzyme was functionally and structurally characterized and was shown to have two redox active sites: a covalently bound FAD and an adjacent pair of cysteine residues. Most interestingly, the X-ray structure revealed the presence of a chain of three sulfur atoms bridging those two cysteine residues. The possible implications of this observation in the catalytic mechanism for sulfide oxidation are discussed, and the role of SQR in the sulfur dependent bioenergetics of A. ambivalens, linked to oxygen reduction, is addressed. Structural and functional insights into sulfide:quinone oxidoreductase.,Brito JA, Sousa FL, Stelter M, Bandeiras TM, Vonrhein C, Teixeira M, Pereira MM, Archer M Biochemistry. 2009 Jun 23;48(24):5613-22. PMID:19438211 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Quinone reductase 3D structures

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