3iir

Crystal Structure of Miraculin like protein from seeds of Murraya koenigii

spinqualitylabelsall models 3iir, resolution 2.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3iir is a 2 chain structure with sequence from Murraya koenigii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.9Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function D2YW43_MURKO Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Earlier, the purification of a 21.4kDa protein with trypsin inhibitory activity from seeds of Murraya koenigii has been reported. The present study, based on the amino acid sequence deduced from both cDNA and genomic DNA, establishes it to be a miraculin-like protein and provides crystal structure at 2.9A resolution. The mature protein consists of 190 amino acid residues with seven cysteines arranged in three disulfide bridges. The amino acid sequence showed maximum homology and formed a distinct cluster with miraculin-like proteins, a soybean Kunitz super family member, in phylogenetic analyses. The major differences in sequence were observed at primary and secondary specificity sites in the reactive loop when compared to classical Kunitz family members. The crystal structure analysis showed that the protein is made of twelve antiparallel beta-strands, loops connecting beta-strands and two short helices. Despite similar overall fold, it showed significant differences from classical Kunitz trypsin inhibitors. Cloning, sequence analysis and crystal structure determination of a miraculin-like protein from Murraya koenigii.,Gahloth D, Selvakumar P, Shee C, Kumar P, Sharma AK Arch Biochem Biophys. 2010 Feb 1;494(1):15-22. Epub 2009 Nov 13. PMID:19914199 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Miraculin Trypsin inhibitor 3D structures

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