3jty

Crystal structure of a BenF-like porin from Pseudomonas fluorescens Pf-5

spinqualitylabelsall models 3jty, resolution 2.58Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3jty is a 4 chain structure with sequence from Pseudomonas protegens Pf-5. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.58Å Ligands: LDA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q4KH25_PSEF5 Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The X-ray structure of a putative BenF-like (gene name: PFL1329) protein from Pseudomonas fluorescens Pf-5 (PflBenF) has been determined at 2.6A resolution. X-ray crystallography revealed a canonical 18-stranded beta-barrel fold that forms a central pore with a diameter of approximately 4.6A, which is consistent with the size and physicochemical properties of the presumed aromatic acid substrate, benzoate. Detailed comparisons with the previously-determined structure of Pseudomonas aeruginosa OpdK, a vanillate influx channel, revealed an arginine-rich aromatic acid selectivity filter of nearly identical structure composed of seven highly conserved residues Arg approximately Asp approximately Arg approximately Arg approximately Ser approximately Asp approximately Arg (R approximately D approximately R approximately R approximately S approximately D approximately R sequence motif, where approximately denotes intervening residues) that define the narrowest part of the pore. Structure of a putative BenF-like porin from Pseudomonas fluorescens Pf-5 at 2.6 A resolution.,Sampathkumar P, Lu F, Zhao X, Li Z, Gilmore J, Bain K, Rutter ME, Gheyi T, Schwinn KD, Bonanno JB, Pieper U, Fajardo JE, Fiser A, Almo SC, Swaminathan S, Chance MR, Baker D, Atwell S, Thompson DA, Emtage JS, Wasserman SR, Sali A, Sauder JM, Burley SK Proteins. 2010 Nov 1;78(14):3056-62. doi: 10.1002/prot.22829. PMID:20737437 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Porin 3D structures

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