3k1b

Structure of OmpF porin

spinqualitylabelsall models 3k1b, resolution 4.39Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3k1b is a 4 chain structure with sequence from Escherichia coli BL21(DE3). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 4.39Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function OMPF_ECOLI Forms pores that allow passive diffusion of small molecules across the outer membrane. It is also a receptor for the bacteriophage T2. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The endogenous Escherichia coli porin OmpF was crystallized as an accidental by-product of our efforts to express, purify and crystallize the Escherichia coli integral membrane protein KdpD in the presence of foscholine-12. Foscholine-12 is widely used in membrane protein studies, but no crystal structure of a protein that was both purified and crystallized with this detergent has been reported in PDB. Crystallization screening for KdpD yielded two different crystals of contaminating protein OmpF. Here we report two OmpF structures, the first membrane protein crystal structures for which extraction, purification and crystallization were done exclusively with foscholine-12. The first structure was refined in space group P21 with cell parameters a = 136.7 A, b = 210.5 A, c = 137 A and beta = 100.5 degrees , and the resolution of 3.8 A. The second structure was solved at the resolution of 4.4 A and was refined in the P321 space group, with unit-cell parameters a = 215.5 A, b = 215.5 A, c = 137.5 A and gamma = 120 degrees . Both crystal forms show novel crystal packing, in which the building block is a tetrahedral arrangement of four trimers. Additionally we discuss the use of foscholine-12 for membrane protein crystallization and structure determination, as well as the problem of the OmpF contamination for membrane proteins overexpressed in E. coli. Structures of the OmpF porin crystallized in the presence of foscholine-12.,Kefala G, Ahn C, Krupa M, Esquivies L, Maslennikov I, Kwiatkowski W, Choe S Protein Sci. 2010 Mar 1. PMID:20196071 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Porin 3D structures

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