3mj8

Crystal structure of HL4E10 Fab, a hamster Ab stimulatory for gammadelta T cells

spinqualitylabelsall models 3mj8, resolution 2.94Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3mj8 is a 4 chain structure with sequence from Nothocricetulus migratorius. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.94Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Hamsters are widely used to generate monoclonal antibodies against mouse, rat, and human antigens, but sequence and structural information for hamster immunoglobulins is sparse. To our knowledge, only three hamster IgG sequences have been published, all of which use kappa light chains, and no three-dimensional structure of a hamster antibody has been reported. We generated antibody HL4E10 as a probe to identify novel costimulatory molecules on the surface of gammadelta T cells which lack the traditional alphabeta T cell co-receptors CD4, CD8, and the costimulatory molecule CD28. HL4E10 binding to gammadelta T cell, surface-expressed, Junctional Adhesion Molecule-Like (JAML) protein leads to potent costimulation via activation of MAP kinase pathways and cytokine production, resulting in cell proliferation. The cDNA sequence of HL4E10 is the first example of a hamster lambda light chain and only the second known complete hamster heavy chain sequence. The crystal structure of the HL4E10 Fab at 2.95 A resolution reveals a rigid combining site with pockets faceted by solvent-exposed tyrosine residues, which are structurally optimized for JAML binding. The characterization of HL4E10 thus comprises a valuable addition to the spartan database of hamster immunoglobulin genes and structures. As the HL4E10 antibody is uniquely costimulatory for gammadelta T cells, humanized versions thereof may be of clinical relevance in treating gammadelta T cell dysfunction-associated diseases, such as chronic non-healing wounds and cancer. cDNA sequence and Fab crystal structure of HL4E10, a hamster IgG lambda light chain antibody stimulatory for gammadelta T cells.,Verdino P, Witherden DA, Podshivalova K, Rieder SE, Havran WL, Wilson IA PLoS One. 2011;6(5):e19828. doi: 10.1371/journal.pone.0019828. Epub 2011 May 24. PMID:21629689 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Antibody 3D structures Sandbox 20009 3D structures of non-human antibody

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