3ngg

X-ray Structure of Omwaprin

spinqualitylabelsall models 3ngg, resolution 1.33Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3ngg is a 2 chain structure with sequence from Oxyuranus microlepidotus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.33Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function WAPA_OXYMI Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The 50-residue snake venom protein L-omwaprin and its enantiomer D-omwaprin were prepared by total chemical synthesis. Radial diffusion assays were performed against Bacillus megaterium and Bacillus anthracis; both L-omwaprin and D-omwaprin showed anti-bacterial activity against B. megaterium. The native protein enantiomer, made of L- amino acids, failed to crystallize readily. However, when a racemic mixture containing equal amounts of L-omwaprin and D-omwaprin was used, diffraction quality crystals were obtained. The racemic protein sample crystallized in the centrosymmetric space group P2(1)/c and its structure was determined at atomic resolution (1.33 A) by a combination of Patterson and direct methods based on the strong scattering from the sulfur atoms in the eight cysteine residues per protein. Racemic crystallography once again proved to be a valuable method for obtaining crystals of recalcitrant proteins, and for determining high resolution X-ray structures by direct methods. Determination of the X-ray structure of the snake venom protein omwaprin by total chemical synthesis and racemic protein crystallography.,Banigan JR, Mandal K, Sawaya MR, Thammavongsa V, Hendrickx AP, Schneewind O, Yeates TO, H Kent SB Protein Sci. 2010 Jul 28. PMID:20669184 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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