3oba

Structure of the beta-galactosidase from Kluyveromyces lactis

spinqualitylabelsall models 3oba, resolution 2.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3oba is a 4 chain structure with sequence from Kluyveromyces lactis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.75Å Ligands: GOL, MN3 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function BGAL_KLULA Publication Abstract from PubMed beta-Galactosidase or lactase is a very important enzyme in the food industry, being that from the yeast Kluyveromyces lactis the most widely used. Here we report its three-dimensional structure both in the free state and complexed with the product galactose. The monomer folds into five domains in a pattern conserved with the prokaryote enzymes of the GH2 family, although two long insertions in domains 2 and 3 are unique and related to oligomerization and specificity. The tetrameric enzyme is a dimer of dimers, with higher dissociation energy for the dimers than for its assembly. Two active centers are located at the interface within each dimer in a narrow channel. The insertion at domain 3 protrudes into this channel and makes putative links with the aglycone moiety of docked lactose. In spite of common structural features related to function, the determinants of the reaction mechanism proposed for Escherichia coli beta-galactosidase are not found in the active site of the K. lactis enzyme. This is the first X-ray crystal structure for a beta-galactosidase used in food processing. Structural basis of specificity in tetrameric Kluyveromyces lactis beta-galactosidase.,Pereira-Rodriguez A, Fernandez-Leiro R, Gonzalez-Siso MI, Cerdan ME, Becerra M, Sanz-Aparicio J J Struct Biol. 2011 Dec 13. PMID:22193516 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Galactosidase 3D structures

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