3pj7

Crystal structure of far-red fluorescent protein Katushka crystallized at pH 8.5

spinqualitylabelsall models 3pj7, resolution 1.85Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3pj7 is a 4 chain structure with sequence from Entacmaea quadricolor. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.85Å Ligands: NRQ Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function H3JQU6_ENTQU Publication Abstract from PubMed The wild type red fluorescent protein eqFP578 (from sea anemone Entacmaea quadricolor, lambdaex = 552 nm, lambdaem = 578 nm) and its bright far-red fluorescent variant Katushka (lambdaex = 588 nm, lambdaem = 635 nm) are characterized by the pronounced pH dependence of their fluorescence. The crystal structures of eqFP578f (eqFP578 with two point mutations improving the protein folding) and Katushka have been determined at the resolution ranging from 1.15 to 1.85 A at two pH values, corresponding to low and high level of fluorescence. The observed extinguishing of fluorescence upon reducing pH in eqFP578f and Katushka has been shown to be accompanied by the opposite trans-cis and cis-trans chromophore isomerization, respectively. Asn143, Ser158, His197 and Ser143, Leu174, and Arg197 have been shown to stabilize the respective trans and cis fluorescent states of the chromophores in eqFP578f and Katushka at higher pH. The cis state has been suggested as being primarily responsible for the observed far-red shift of the emission maximum of Katushka relative to that of eqFP578f. Crystallographic study of red fluorescent protein eqFP578 and its far-red variant Katushka reveals opposite pH-induced isomerization of chromophore.,Pletneva NV, Pletnev VZ, Shemiakina II, Chudakov DM, Artemyev I, Wlodawer A, Dauter Z, Pletnev S Protein Sci. 2011 May 11. doi: 10.1002/pro.654. PMID:21563226 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Green Fluorescent Protein 3D structures

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