3qdt

Structure of Boletus edulis lectin in complex with T-antigen disaccharide

spinqualitylabelsall models 3qdt, resolution 1.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3qdt is a 2 chain structure with sequence from Boletus edulis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.3Å Ligands: A2G, ACE, GAL, PRD_900084 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function BEL_BOLED Publication Abstract from PubMed A novel lectin has been isolated from the fruiting bodies of the common edible mushroom Boletus edulis (king bolete, penny bun, porcino, or cep) by affinity chromatography on a chitin column. We propose for the lectin the name BEL (Boletus edulis lectin). BEL inhibits selectively the proliferation of several malignant cell lines and binds the neoplastic cell specific T-antigen disaccharide, Galbeta1-3GalNAc. The lectin was structurally characterized: the molecule is a homotetramer and the 142 amino acid sequence of the chains was determined. The protein belongs to the saline-soluble family of mushroom fruiting body specific lectins. BEL was also crystallized and its three-dimensional structure was determined by X-ray diffraction to 1.15 A resolution. The structure is similar to that of Agaricus bisporus lectin. Using the appropriate co-crystals, the interactions of BEL with specific mono and disaccharides were also studied by X-ray diffraction. The six structures of carbohydrate complexes reported here provide details of the interactions of the ligands with the lectin and shed light on the selectivity of the two distinct binding sites present in each protomer. STRUCTURE OF A LECTIN WITH ANTITUMORAL PROPERTIES IN KING BOLETE (Boletus edulis) MUSHROOMS.,Bovi M, Carrizo ME, Capaldi S, Perduca M, Chiarelli LR, Galliano M, Monaco HL Glycobiology. 2011 Feb 8. PMID:21303815 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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