3qz0

Structure of Treponema denticola Factor H Binding protein (FhbB), selenomethionine derivative

spinqualitylabelsall models 3qz0, resolution 1.77Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3qz0 is a 2 chain structure with sequence from Treponema denticola. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.77Å Ligands: GOL, MSE, SCN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Periodontitis is the most common disease of microbial etiology in humans. Periopathogen survival is dependent upon evasion of complement-mediated destruction. Treponema denticola, an important contributor to periodontitis, evades killing by the alternative complement cascade by binding factor H (FH) to its surface. Bound FH is rapidly cleaved by the T. denticola protease, dentilisin. In this report, the structure of the T. denticola FH-binding protein, FhbB, was solved to 1.7 A resolution. FhbB possesses a unique fold that imparts high thermostability. The kinetics of the FH/FhbB interaction were assessed using surface plasmon resonance. A K(D) value in the micromolar range (low affinity) was demonstrated, and rapid off kinetics were observed. Site-directed mutagenesis and sucrose octasulfate competition assays collectively indicate that the negatively charged face of FhbB binds within FH complement control protein module 7. This study provides significant new insight into the molecular basis of FH/FhbB interaction and advances our understanding of the role that T. denticola plays in the development and progression of periodontal disease. Structure of Factor H-binding Protein B (FhbB) of the Periopathogen, Treponema denticola: INSIGHTS INTO PROGRESSION OF PERIODONTAL DISEASE.,Miller DP, Bell JK, McDowell JV, Conrad DH, Burgner JW, Heroux A, Marconi RT J Biol Chem. 2012 Apr 13;287(16):12715-22. Epub 2012 Feb 24. PMID:22371503 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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