3u6z

Crystal structure of the complex formed between type 1 ribosome inactivating protein and adenine at 1.7A resolution

spinqualitylabelsall models 3u6z, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3u6z is a 1 chain structure with sequence from Momordica balsamina. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.7Å Ligands: ADE, GOL, NAG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function D9J2T9_MOMBA Publication Abstract from PubMed The ribosome inactivating proteins (RIPs) of type 1 are plant toxins that eliminate adenine base selectively from the single stranded loop of rRNA. We report six crystal structures, type 1 RIP from Momordica balsamina (A), three in complexed states with ribose (B), guanine (C) and adenine (D) and two structures of MbRIP-1 when crystallized with adenosine triphosphate (ATP) (E) and 2'-deoxyadenosine triphosphate (2'-dATP) (F). These were determined at 1.67A, 1.60A, 2.20A, 1.70A, 2.07A and 1.90A resolutions respectively. The structures contained, (A) unbound protein molecule, (B) one protein molecule and one ribose sugar, (C) one protein molecule and one guanine base, (D) one protein molecule and one adenine base, (E) one protein molecule and one ATP-product adenine molecule and (F) one protein molecule and one 2'-dATP-product adenine molecule. Three distinct conformations of the side chain of Tyr70 were observed with (i) chi(1)=-66 degrees and chi(2)=165 degrees in structures (A) and (B); (ii) chi(1)=-95 degrees and chi(2)=70 degrees in structures (C), (D) and (E); and (iii) chi(1)=-163 degrees and chi(2)=87 degrees in structure (F). The conformation of Tyr70 in (F) corresponds to the structure of a conformational intermediate. This is the first structure which demonstrates that the slow conversion of DNA substrates by RIPs can be trapped during crystallization. Crystal structures of a type-1 ribosome inactivating protein from Momordica balsamina in the bound and unbound states.,Kushwaha GS, Pandey N, Sinha M, Singh SB, Kaur P, Sharma S, Singh TP Biochim Biophys Acta. 2012 Apr;1824(4):679-91. Epub 2012 Feb 14. PMID:22361570 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosome inactivating protein 3D structures

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