3vv2
Crystal structure of complex form between S324A-subtilisin and mutant Tkpro
Structural highlights
FunctionTKSU_THEKO Has a broad substrate specificity with a slight preference to large hydrophobic amino acid residues at the P1 position. Publication Abstract from PubMedTk-subtilisin, a subtilisin homologue (Gly70-Gly398) from Thermococcus kodakarensis, is matured from its precursor, Pro-Tk-subtilisin [Tk-subtilisin in a pro form (Gly1-Gly398)], by autoprocessing and degradation of propeptide [Tk-propeptide, a propeptide of Tk-subtilisin (Gly1-Leu69)]. The scissile peptide bond between Leu69 and Gly70 of Pro-Tk-subtilisin is first self-cleaved to produce an inactive Tk-propeptide:Tk-subtilisin complex, in which the C-terminal region of Tk-propeptide binds to the active-site cleft of Tk-subtilisin. Tk-propeptide is then dissociated from Tk-subtilisin and degraded by Tk-subtilisin to release active Tk-subtilisin. To examine whether the mutation of Leu69 to Pro, which is the most unfavourable residue in the P1 position for subtilisins, affects the maturation of Pro-Tk-subtilisin, the Pro-Tk-subtilisin and Tk-propeptide derivatives with this mutation (Pro-L69P and L69P-propeptide) were constructed and characterized. Pro-L69P was autoprocessed more slowly than Pro-Tk-subtilisin. Nevertheless, it matured to Tk-subtilisin more rapidly than Pro-Tk-subtilisin because L69P-propeptide was degraded by Tk-subtilisin more rapidly than Tk-propeptide. The chaperone function and stability of L69P-propeptide were comparable to those of Tk-propeptide, whereas the inhibitory potency and binding ability of L69P-propeptide were considerably reduced compared to those of Tk-propeptide. The crystal structure of the complex between L69P-propeptide and S324A-subtilisin (i.e. a protease activity-defective mutant) revealed that the C-terminal region of L69P-propeptide does not well fit into the substrate binding pockets of Tk-subtilisin (S1-S4 subsites) as a result of a conformational change caused by the mutation. These results suggest that the Leu-->Pro mutation accelerates the maturation of Pro-Tk-subtilisin by reducing the binding ability of Tk-propeptide to Tk-subtilisin. DATABASE: The coordinates and structure factors have been deposited in the RCSB Protein Data Bank under ID code: 3VV2. STRUCTURED DIGITAL ABSTRACT: Pro-Tk-subtilisin and Pro-Tk-subtilisin cleave by enzymatic study (View interaction) L69P-propeptide and S324A-subtilisin bind by x-ray crystallography (View interaction) Tk-propeptide binds to S324A-subtilisin by surface plasmon resonance (View interaction). Accelerated maturation of Tk-subtilisin by a Leu-->Promutation at the C-terminus of the propeptide, which reduces the binding of the propeptide to Tk-subtilisin.,Uehara R, Ueda Y, You DJ, Koga Y, Kanaya S FEBS J. 2013 Feb;280(4):994-1006. doi: 10.1111/febs.12091. Epub 2013 Jan 11. PMID:23237738[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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