3wpx

Structure of PomBc4, a periplasmic fragment of PomB from Vibrio alginolyticus

spinqualitylabelsall models 3wpx, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3wpx is a 2 chain structure with sequence from Vibrio alginolyticus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.3Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function O06874_VIBAL Publication Abstract from PubMed The torque of the bacterial flagellum is generated by the rotor-stator interaction coupled with the ion flow through the channel in the stator. Anchoring the stator unit to the peptidoglycan layer with proper orientation around the rotor is believed to be essential for smooth rotation of the flagellar motor. The stator unit of the sodium-driven flagellar motor of Vibrio is composed of PomA and PomB, and is thought to be fixed to the peptidoglycan layer and the T-ring by the C-terminal periplasmic region of PomB. Here, we report the crystal structure of a C-terminal fragment of PomB (PomBC) at 2.0-A resolution, and the structure suggests a conformational change in the N-terminal region of PomBC for anchoring the stator. On the basis of the structure, we designed double-Cys replaced mutants of PomB for in vivo disulfide cross-linking experiments and examined their motility. The motility can be controlled reproducibly by reducing reagent. The results of these experiments suggest that the N-terminal disordered region (121-153) and following the N-terminal two-thirds of alpha1(154-164) in PomBC changes its conformation to form a functional stator around the rotor. The cross-linking did not affect the localization of the stator nor the ion conductivity, suggesting that the conformational change occurs in the final step of the stator assembly around the rotor. Conformational change in the periplamic region of the flagellar stator coupled with the assembly around the rotor.,Zhu S, Takao M, Li N, Sakuma M, Nishino Y, Homma M, Kojima S, Imada K Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13523-8. doi:, 10.1073/pnas.1324201111. Epub 2014 Sep 2. PMID:25197056 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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