3wvj

The crystal structure of native glycosidic hydrolase

spinqualitylabelsall models 3wvj, resolution 1.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3wvj is a 2 chain structure with sequence from Acetivibrio thermocellus ATCC 27405. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.95Å Ligands: B3P Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function GUB_ACET2 Publication Abstract from PubMed A thermophilic glycoside hydrolase family 16 (GH16) beta-1,3-1,4-glucanase from Clostridium thermocellum (CtLic16A) holds great potentials in industrial applications due to its high specific activity and outstanding thermostability. In order to understand its molecular machinery, the crystal structure of CtLic16A was determined to 1.95A resolution. The enzyme folds into a classic GH16 beta-jellyroll architecture which consists of two beta-sheets atop each other, with the substrate-binding cleft lying on the concave side of the inner beta-sheet. Two Bis-Tris propane molecules were found in the positive and negative substrate binding sites. Structural analysis suggests that the major differences between the CtLic16A and other GH16 beta-1,3-1,4-glucanase structures occur at the protein exterior. Furthermore, the high catalytic efficacy and thermal profile of the CtLic16A are preserved in the enzyme produced in Pichia pastoris, encouraging its further commercial applications. Structural analyses and yeast production of the beta-1,3-1,4-glucanase catalytic module encoded by the licB gene of Clostridium thermocellum.,Chen CC, Huang JW, Zhao P, Ko TP, Huang CH, Chan HC, Huang Z, Liu W, Cheng YS, Liu JR, Guo RT Enzyme Microb Technol. 2015 Apr;71:1-7. doi: 10.1016/j.enzmictec.2015.01.002., Epub 2015 Jan 19. PMID:25765303 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Glucanase 3D structures

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