3wvk

Time-Resolved Crystal Structure of HindIII with 230sec soaking

spinqualitylabelsall models 3wvk, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3wvk is a 16 chain structure with sequence from Haemophilus influenzae Rd KW20 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2Å Ligands: GOL, MN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function T2D3_HAEIN Recognizes the double-stranded sequence AAGCTT and cleaves after A-1. Publication Abstract from PubMed In order to investigate the mechanism of the reaction catalyzed by HindIII, structures of HindIII-DNA complexes with varying durations of soaking time in cryoprotectant buffer containing manganese ions were determined by the freeze-trap method. In the crystal structures of the complexes obtained after soaking for a longer duration, two manganese ions, indicated by relatively higher electron density, are clearly observed at the two metal ion-binding sites in the active site of HindIII. The increase in the electron density of the two metal-ion peaks followed distinct pathways with increasing soaking times, suggesting variation in the binding rate constant for the two metal sites. DNA cleavage is observed when the second manganese ion appears, suggesting that HindIII uses the two-metal-ion mechanism, or alternatively that its reactivity is enhanced by the binding of the second metal ion. In addition, conformational change in a loop near the active site accompanies the catalytic reaction. Analysis of the HindIII-catalyzed reaction by time-resolved crystallography.,Kawamura T, Kobayashi T, Watanabe N Acta Crystallogr D Biol Crystallogr. 2015 Feb;71(Pt 2):256-65. doi:, 10.1107/S1399004714025188. Epub 2015 Jan 23. PMID:25664735 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Endonuclease 3D structures

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