3zh9

Bacillus subtilis DNA clamp loader delta protein (YqeN)

spinqualitylabelsall models 3zh9, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3zh9 is a 1 chain structure with sequence from Bacillus subtilis subsp. subtilis str. 168. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.1Å Ligands: GOL, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function YQEN_BACSU Publication Abstract from PubMed The clamp-loader complex plays a crucial role in DNA replication by loading the beta-clamp onto primed DNA to be used by the replicative polymerase. Relatively little is known about the stoichiometry, structure and assembly pathway of this complex, and how it interacts with the replicative helicase, in Gram-positive organisms. Analysis of full and partial complexes by mass spectrometry revealed that a hetero-pentameric tau3-delta-delta' Bacillus subtilis clamp-loader assembles via multiple pathways, which differ from those exhibited by the Gram-negative model Escherichia coli. Based on this information, a homology model of the B. subtilis tau3-delta-delta' complex was constructed, which revealed the spatial positioning of the full C-terminal tau domain. The structure of the delta subunit was determined by X-ray crystallography and shown to differ from that of E. coli in the nature of the amino acids comprising the tau and delta' binding regions. Most notably, the tau-delta interaction appears to be hydrophilic in nature compared with the hydrophobic interaction in E. coli. Finally, the interaction between tau3 and the replicative helicase DnaB was driven by ATP/Mg2+ conformational changes in DnaB, and evidence is provided that hydrolysis of one ATP molecule by the DnaB hexamer is sufficient to stabilize its interaction with tau3. Insights into the structure and assembly of the Bacillus subtilis clamp-loader complex and its interaction with the replicative helicase.,Afonso JP, Chintakayala K, Suwannachart C, Sedelnikova S, Giles K, Hoyes JB, Soultanas P, Rafferty JB, Oldham NJ Nucleic Acids Res. 2013 Mar 21. PMID:23525462 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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