4aaj

Structure of N-(5'-phosphoribosyl)anthranilate isomerase from Pyrococcus furiosus

spinqualitylabelsall models 4aaj, resolution 1.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4aaj is a 1 chain structure with sequence from Pyrococcus furiosus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.75Å Ligands: SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function TRPF_PYRFU Publication Abstract from PubMed The structure of phosphoribosyl anthranilate isomerase (TrpF) from the hyperthermophilic archaeon Pyrococcus furiosus (PfTrpF) has been determined at 1.75 A resolution. The PfTrpF structure has a monomeric TIM-barrel fold which differs from the dimeric structures of two other known thermophilic TrpF proteins. A comparison of the PfTrpF structure with the two known bacterial thermophilic TrpF structures and the structure of a related mesophilic protein from Escherichia coli (EcTrpF) is presented. The thermophilic TrpF structures contain a higher proportion of ion pairs and charged residues compared with the mesophilic EcTrpF. These residues contribute to the closure of the central barrel and the stabilization of the barrel and the surrounding alpha-helices. In the monomeric PfTrpF conserved structural water molecules are mostly absent; instead, the structural waters are replaced by direct side-chain-main-chain interactions. As a consequence of these combined mechanisms, the P. furiosus enzyme is a thermodynamically stable and entropically optimized monomeric TIM-barrel enzyme which defines a good framework for further protein engineering for industrial applications. A monomeric TIM-barrel structure from Pyrococcus furiosus is optimized for extreme temperatures.,Repo H, Oeemig JS, Djupsjobacka J, Iwai H, Heikinheimo P Acta Crystallogr D Biol Crystallogr. 2012 Nov;68(Pt 11):1479-87. doi:, 10.1107/S0907444912037171. Epub 2012 Oct 13. PMID:23090397 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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