4alz

The Yersinia T3SS basal body component YscD reveals a different structural periplasmatic domain organization to known homologue PrgH

spinqualitylabelsall models 4alz, resolution 1.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4alz is a 1 chain structure with sequence from Yersinia enterocolitica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.4Å Ligands: GOL, PO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function YSCD_YEREN Required for the export process of the Yop proteins. Publication Abstract from PubMed Injectisomes are multi-protein transmembrane machines allowing pathogenic bacteria to inject effector proteins into eukaryotic host cells, a process called type III secretion. Here we present the first three-dimensional structure of Yersinia enterocolitica and Shigella flexneri injectisomes in situ and the first structural analysis of the Yersinia injectisome. Unexpectedly, basal bodies of injectisomes inside the bacterial cells showed length variations of 20%. The in situ structures of the Y. enterocolitica and S. flexneri injectisomes had similar dimensions and were significantly longer than the isolated structures of related injectisomes. The crystal structure of the inner membrane injectisome component YscD appeared elongated compared to a homologous protein, and molecular dynamics simulations documented its elongation elasticity. The ring-shaped secretin YscC at the outer membrane was stretched by 30-40% in situ, compared to its isolated liposome-embedded conformation. We suggest that elasticity is critical for some two-membrane spanning protein complexes to cope with variations in the intermembrane distance. DOI:https://dx.doi.org/10.7554/eLife.00792.001. In situ structural analysis of the Yersinia enterocolitica injectisome.,Kudryashev M, Stenta M, Schmelz S, Amstutz M, Wiesand U, Castano-Diez D, Degiacomi MT, Munnich S, Bleck CK, Kowal J, Diepold A, Heinz DW, Dal Peraro M, Cornelis GR, Stahlberg H Elife. 2013 Jul 30;2:e00792. doi: 10.7554/eLife.00792. Print 2013. PMID:23908767 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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