4bkk

The Respiratory Syncytial Virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid.

Loading... Status: waiting for Mol* initialization Structural highlights 4bkk is a 24 chain structure with sequence from Human respiratory syncytial virus A strain Long. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function NCAP_HRSVA Encapsidates the genome, protecting it from nucleases. The nucleocapsid (NC) has a helical structure. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by protein N is coupled to RNA synthesis and all replicative products are resistant to nucleases. Publication Abstract from PubMed Respiratory syncytial virus (RSV) is an important human pathogen. Its nucleocapsid (NC), which comprises the negative sense RNA viral genome coated by the viral nucleoprotein N, is a critical assembly that serves as template for both mRNA synthesis and genome replication. We have previously described the X-ray structure of an NC-like structure: a decameric ring formed of N-RNA that mimics one turn of the helical NC. In the absence of experimental data we had hypothesized that the NC helix would be right-handed, as the N-N contacts in the ring appeared to more easily adapt to that conformation. We now unambiguously show that the RSV NC is a left-handed helix. We further show that the contacts in the ring can be distorted to maintain key N-N-protein interactions in a left-handed helix, and discuss the implications of the resulting atomic model of the helical NC for viral replication and transcription. The respiratory syncytial virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid.,Bakker SE, Duquerroy S, Galloux M, Loney C, Conner E, Eleouet JF, Rey FA, Bhella D J Gen Virol. 2013 Aug;94(Pt 8):1734-8. doi: 10.1099/vir.0.053025-0. Epub 2013 May, 15. PMID:23677789 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Nucleoprotein 3D structures

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