4c0r

Molecular and structural basis of glutathione import in Gram-positive bacteria via GshT and the cystine ABC importer TcyBC of Streptococcus mutans.

spinqualitylabelsall models 4c0r, resolution 1.55Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4c0r is a 2 chain structure with sequence from Streptococcus mutans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.547Å Ligands: CD, CL, GDS Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q93DA5_STRMG Publication Abstract from PubMed Glutathione (GSH) protects cells against oxidative injury and maintains a range of vital functions across all branches of life. Despite recent advances in our understanding of the transport mechanisms responsible for maintaining the spatiotemporal homeostasis of GSH and its conjugates in eukaryotes and Gram-negative bacteria, the molecular and structural basis of GSH import into Gram-positive bacteria has remained largely uncharacterized. Here, we employ genetic, biochemical and structural studies to investigate a possible glutathione import axis in Streptococcus mutans, an organism that has hitherto served as a model system. We show that GshT, a type 3 solute binding protein, displays physiologically relevant affinity for GSH and glutathione disulfide (GSSG). The crystal structure of GshT in complex with GSSG reveals a collapsed structure whereby the GS-I-leg of GSSG is accommodated tightly via extensive interactions contributed by the N- and C-terminal lobes of GshT, while the GS-II leg extends to the solvent. This can explain the ligand promiscuity of GshT in terms of binding glutathione analogues with substitutions at the cysteine-sulfur or the glycine-carboxylate. Finally, we show that GshT primes glutathione import via the l-cystine ABC transporter TcyBC, a membrane permease, which had previously exclusively been associated with the transport of l-cystine. Molecular and structural basis of glutathione import in Gram-positive bacteria via GshT and the cystine ABC importer TcyBC of Streptococcus mutans.,Vergauwen B, Verstraete K, Senadheera DB, Dansercoer A, Cvitkovitch DG, Guedon E, Savvides SN Mol Microbiol. 2013 Jul;89(2):288-303. doi: 10.1111/mmi.12274. Epub 2013 Jun 10. PMID:23701283 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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