4cc8

Pre-fusion structure of trimeric HIV-1 envelope glycoprotein determined by cryo-electron microscopy

Loading... Status: waiting for Mol* initialization Structural highlights 4cc8 is a 12 chain structure with sequence from Homo sapiens and Human immunodeficiency virus 1. The January 2014 RCSB PDB Molecule of the Month feature on HIV Envelope Glycoprotein by David Goodsell is 10.2210/rcsb_pdb/mom_2014_1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 6Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The activation of trimeric HIV-1 envelope glycoprotein (Env) by its binding to the cell-surface receptor CD4 and co-receptors (CCR5 or CXCR4) represents the first of a series of events that lead to fusion between viral and target-cell membranes. Here, we present the cryo-EM structure, at subnanometer resolution ( approximately 6 A at 0.143 FSC), of the 'closed', prefusion state of trimeric HIV-1 Env complexed to the broadly neutralizing antibody VRC03. We show that three gp41 helices at the core of the trimer serve as an anchor around which the rest of Env is reorganized upon activation to the 'open' quaternary conformation. The architecture of trimeric HIV-1 Env in the prefusion state and in the activated intermediate state resembles the corresponding states of influenza hemagglutinin trimers, thus providing direct evidence for the similarity in entry mechanisms used by HIV-1, influenza and related enveloped viruses. Prefusion structure of trimeric HIV-1 envelope glycoprotein determined by cryo-electron microscopy.,Bartesaghi A, Merk A, Borgnia MJ, Milne JL, Subramaniam S Nat Struct Mol Biol. 2013 Oct 23. doi: 10.1038/nsmb.2711. PMID:24154805 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Art:HIV Glycoprotein Gp120 3D structures Gp41 3D Structures Monoclonal Antibodies 3D structures

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