4ci0

Electron cryo-microscopy of F420-reducing NiFe hydrogenase Frh

Loading... Status: waiting for Mol* initialization Structural highlights 4ci0 is a 3 chain structure with sequence from Methanothermobacter marburgensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.36Å Ligands: FAD, FE, FE2, NI, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function D9PYF9_METTM Publication Abstract from PubMed The introduction of direct electron detectors with higher detective quantum efficiency and fast read-out marks the beginning of a new era in electron cryo-microscopy. Using the FEI Falcon II direct electron detector in video mode, we have reconstructed a map at 3.36 A resolution of the 1.2 MDa F420-reducing hydrogenase (Frh) from methanogenic archaea from only 320,000 asymmetric units. Videos frames were aligned by a combination of image and particle alignment procedures to overcome the effects of beam-induced motion. The reconstructed density map shows all secondary structure as well as clear side chain densities for most residues. The full coordination of all cofactors in the electron transfer chain (a [NiFe] center, four [4Fe4S] clusters and an FAD) is clearly visible along with a well-defined substrate access channel. From the rigidity of the complex we conclude that catalysis is diffusion-limited and does not depend on protein flexibility or conformational changes. DOI: https://dx.doi.org/10.7554/eLife.01963.001. Atomic model of the F420-reducing [NiFe] hydrogenase by electron cryo-microscopy using a direct electron detector.,Allegretti M, Mills DJ, McMullan G, Kuhlbrandt W, Vonck J Elife. 2014 Feb 25;3:e01963. doi: 10.7554/eLife.01963. PMID:24569482 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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