4dlc

Crystal Structure of Trypanosoma brucei dUTPase with dUMP, MgF3- transition state analogue, and Mg2+

spinqualitylabelsall models 4dlc, resolution 1.76Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4dlc is a 1 chain structure with sequence from Trypanosoma brucei. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.759Å Ligands: MG, MGF, UMP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q57ZH3_TRYB2 Publication Abstract from PubMed The Tritryps Trypanosoma brucei, T. cruzi and Leishmania donovani are responsible for great morbidity and mortality in developing countries. Their dimeric dUTPases are members of the all-a NTP pyrophosphohydrolase family and represent promising drug targets due to their essential nature and markedly different structural and biochemical properties compared to the trimeric human enzyme. Here we present the structure of the T. brucei enzyme in open and closed conformations. Furthermore, we probe the reaction mechanism through the binding of transition state mimics both in solution and in the crystal. 31P-NMR and tryptophan fluorescence quenching in the presence of AlF3 and MgF3- identified which phosphate is subject to nucleophilic attack by a water molecule. The structures in complex with two transition state analogues confirm that the nucleophilic attack occurs on the b-phosphate in contrast to the a-phosphate in the trimeric enzymes. These results establish the structural basis of catalysis of these important house-keeping enzymes and has ramifications for the wider all-a NTP pyrophosphohydrolase family. On the catalytic mechanism of dimeric dUTPases.,Hemsworth GR, Gonzalez-Pacanowska D, Wilson KS Biochem J. 2013 Sep 4. PMID:24001052 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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