4dm0

TN5 transposase: 20MER OUTSIDE END 2 MN complex

spinqualitylabelsall models 4dm0, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4dm0 is a 3 chain structure with sequence from Escherichia coli. This structure supersedes the now removed PDB entries 1mur and 1l1a. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.5Å Ligands: EDO, MN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function TN5P_ECOLX Mediates transposition of transposon Tn5 by a 'cut and paste' mechanism. First, the monomeric transposase binds the 19 bp inverted DNA repeats flanking the transposon. Then, dimerization of the DNA-bound transposase creates a synaptic DNA complex. After nicking of the first DNA strand, excision of the transposon proceeds through a series of intermediates. The transposase then mediates the insertion of the transposon at a new site by strand transfer. The activity of the wild-type transposase is very low, and is further inhibited by dimerization with the transposase inhibitor (inh). See Also Transposase 3D structures

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