4fxi | pdb_00004fxi

Crystal structure of the isolated E. coli RelE toxin, P21 form

spinqualitylabelsall models 4fxi, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4fxi is a 3 chain structure with sequence from Escherichia coli K-12. This structure supersedes the now removed PDB entry 3kha. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.8003Å Ligands: CME, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RELE_ECOLI Toxic component of a toxin-antitoxin (TA) module. A sequence-specific, ribosome-dependent mRNA endoribonuclease that inhibits translation during amino acid starvation (the stringent response). Acts by cleaving mRNA with high codon specificity in the ribosomal A site between positions 2 and 3. The stop codon UAG is cleaved at a fast rate while UAA and UGA are cleaved with intermediate and slow rates. mRNA cleavage can also occur in the ribosomal E site after peptide release from peptidyl-tRNA in the P site as well as on free 30S subunits. Overexpression of RelE results in the inhibition of bacterial growth and a sharp decrease in colony-forming ability which is inhibited by the labile cognate antitoxin RelB. Overexpression also sharply increases persisters (cells that neither grow or die in presence of bactericidal agent and are largely responsible for high levels of biofilm tolerance to antimicrobials). Acts with RelB as a corepressor of relBE transcription. Seems to be a principal mediator of cell death in liquid media.

Proteopedia Page Contributors and Editors (what is this?)

OCA