4g37

Structure of cross-linked firefly luciferase in second catalytic conformation

spinqualitylabelsall models 4g37, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4g37 is a 2 chain structure with sequence from Photinus pyralis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.396Å Ligands: CL, SLU, SO4, XLX Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function LUCI_PHOPY Produces green light with a wavelength of 562 nm. Publication Abstract from PubMed Beetle luciferases catalyze a two-step reaction that includes the initial adenylation of the luciferin substrate, followed by an oxidative decarboxylation that ultimately produces light. Evidence for homologous acyl-CoA synthetases supports a domain alternation catalytic mechanism in which these enzymes' C-terminal domain rotates by approximately 140 degrees to adopt two conformations that are used to catalyze the two partial reactions. While many structures exist of acyl-CoA synthetases in both conformations, to date only biochemical evidence supports domain alternation with luciferase. We have determined the structure of a cross-linked luciferase enzyme that is trapped in the second conformation. This new structure supports the role of the second catalytic conformation and provides insights into the biochemical mechanism of the luciferase oxidative step. Crystal Structure of Firefly Luciferase in a Second Catalytic Conformation Supports a Domain Alternation Mechanism.,Sundlov JA, Fontaine DM, Southworth TL, Branchini BR, Gulick AM Biochemistry. 2012 Aug 6. PMID:22852753 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Luciferase 3D structures

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