4g3m

Complex Structure of Bacillus subtilis RibG: The Deamination Process in Riboflavin Biosynthesis

spinqualitylabelsall models 4g3m, resolution 2.56Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4g3m is a 4 chain structure with sequence from Bacillus subtilis subsp. subtilis str. 168. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.56Å Ligands: AI9, AOF, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RIBD_BACSU Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)-pyrimidinedione 5'-phosphate. Publication Abstract from PubMed Eubacterial RibG and yeast Rib2 possess a deaminase domain for pyrimidine deamination in the second and third steps, respectively, of riboflavin biosynthesis. These enzymes are specific for ribose and ribitol, respectively. Here, the crystal structure of Bacillus subtilis RibG in complex with a deaminase product is reported at 2.56 A resolution. Two loops move towards the product on substrate binding, resulting in interactions with the ribosyl and phosphate groups and significant conformational changes. The product carbonyl moiety is bent out of the pyrimidine ring to coordinate to the catalytic zinc ion. Such distortions in the bound substrate and product may play an essential role in enzyme catalysis. The yeast Rib2 structure was modelled and a mutational analysis was carried out in order to understand the mechanism of substrate recognition in these two enzymes. Detailed structural comparisons revealed that the two consecutive carbonyl backbones that occur prior to the PCXXC signature constitute a binding hole for the target amino group of the substrate. This amino-binding hole is essential in B. subtilis RibG and is also conserved in the RNA/DNA-editing deaminases. Evolution of vitamin B(2) biosynthesis: eubacterial RibG and fungal Rib2 deaminases.,Chen SC, Shen CY, Yen TM, Yu HC, Chang TH, Lai WL, Liaw SH Acta Crystallogr D Biol Crystallogr. 2013 Feb;69(Pt 2):227-36. doi:, 10.1107/S0907444912044903. Epub 2013 Jan 19. PMID:23385458 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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