4gg4

Crystal structure of the TAL effector dHax3 bound to specific DNA-RNA hybrid

spinqualitylabelsall models 4gg4, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4gg4 is a 3 chain structure with sequence from Xanthomonas campestris pv. armoraciae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.501Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q3ZD72_XANCA Publication Abstract from PubMed The transcription activator-like (TAL) effector targets specific host promoter through its central DNA-binding domain, which comprises multiple tandem repeats (TALE repeats). Recent structural analyses revealed that the TALE repeats form a superhelical structure that tracks along the forward strand of the DNA duplex. Here, we demonstrate that TALE repeats specifically recognize a DNA-RNA hybrid where the DNA strand determines the binding specificity. The crystal structure of a designed TALE in complex with the DNA-RNA hybrid was determined at a resolution of 2.5 A. Although TALE repeats are in direct contact with only the DNA strand, the phosphodiester backbone of the RNA strand is inaccessible by macromolecules such as RNases. Consistent with this observation, sequence-specific recognition of an HIV-derived DNA-RNA hybrid by an engineered TALE efficiently blocked RNase H-mediated degradation of the RNA strand. Our study broadens the utility of TALE repeats and suggests potential applications in processes involving DNA replication and retroviral infections. Specific DNA-RNA hybrid recognition by TAL effectors.,Yin P, Deng D, Yan C, Pan X, Xi JJ, Yan N, Shi Y Cell Rep. 2012 Oct 25;2(4):707-13. doi: 10.1016/j.celrep.2012.09.001. Epub 2012, Sep 27. PMID:23022487 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also TAL effector

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