4ggz

The structure of bradavidin2-biotin complex

spinqualitylabelsall models 4ggz, resolution 1.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4ggz is a 4 chain structure with sequence from Bradyrhizobium diazoefficiens USDA 110. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.75Å Ligands: BTN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q89U61_BRADU Publication Abstract from PubMed Bradavidin II is a biotin-binding protein from Bradyrhizobium japonicum that resembles chicken avidin and bacterial streptavidin. A biophysical characterization was carried out using dynamic light scattering, native mass spectrometry, differential scanning calorimetry, and isothermal titration calorimetry combined with structural characterization using X-ray crystallography. These observations revealed that bradavidin II differs from canonical homotetrameric avidin protein family members in its quaternary structure. In contrast with the other avidins, bradavidin II appears to have a dynamic (transient) oligomeric state in solution. It is monomeric at low protein concentrations but forms higher oligomeric assemblies at higher concentrations. The crystal structure of bradavidin II revealed an important role for Phe42 in shielding the bound ligand from surrounding water molecules, thus functionally replacing the L7,8 loop essential for tight ligand binding in avidin and streptavidin. This bradavidin II characterization opens new avenues for oligomerization-independent biotin-binding protein development. The highly dynamic oligomeric structure of bradavidin II is unique among avidin proteins.,Leppiniemi J, Meir A, Kahkonen N, Kukkurainen S, Maatta JA, Ojanen M, Janis J, S Kulomaa M, Livnah O, Hytonen VP Protein Sci. 2013 May 10. doi: 10.1002/pro.2281. PMID:23661323 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Avidin 3D structures

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