4h2l

Deer mouse hemoglobin in hydrated format

spinqualitylabelsall models 4h2l, resolution 1.78Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4h2l is a 2 chain structure with sequence from Peromyscus maniculatus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.779Å Ligands: HEM Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A4ZQ95_PERMA Involved in oxygen transport from the lung to the various peripheral tissues (By similarity).[SAAS:SAAS002338_004_005123] Publication Abstract from PubMed The deer mouse, Peromyscus maniculatus, exhibits altitude-associated variation in hemoglobin oxygen affinity. To examine the structural basis of this functional variation, the structure of the hemoglobin was solved. Recombinant hemoglobin was expressed in Escherichia coli and was purified by ion-exchange chromatography. Recombinant hemoglobin was crystallized by the hanging-drop vapor-diffusion method using polyethylene glycol as a precipitant. The obtained orthorhombic crystal contained two subunits in the asymmetric unit. The refined structure was interpreted as the aquo-met form. Structural comparisons were performed among hemoglobins from deer mouse, house mouse and human. In contrast to human hemoglobin, deer mouse hemoglobin lacks the hydrogen bond between alpha1Trp14 in the A helix and alpha1Thr67 in the E helix owing to the Thr67Ala substitution. In addition, deer mouse hemoglobin has a unique hydrogen bond at the alpha1beta1 interface between residues alpha1Cys34 and beta1Ser128. Deer mouse hemoglobin exhibits a lowered oxygen affinity owing to mobility of the E helix.,Inoguchi N, Oshlo JR, Natarajan C, Weber RE, Fago A, Storz JF, Moriyama H Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Apr 1;69(Pt 4):393-8. doi:, 10.1107/S1744309113005708. Epub 2013 Mar 28. PMID:23545644 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Hemoglobin 3D structures

Proteopedia Page Contributors and Editors (what is this?)

OCA