4h79

Crystal structure of CasB from Thermobifida fusca

spinqualitylabelsall models 4h79, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4h79 is a 1 chain structure with sequence from Thermobifida fusca YX. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.9Å Ligands: EDO Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q47PI5_THEFY Publication Abstract from PubMed The CRISPR system is an adaptive RNA-based microbial immune system against invasive genetic elements. CasB is an essential protein component in Type I-E Cascade. Here, we characterize CasB proteins from three different organisms as non-specific nucleic acid binding proteins. The Thermobifida fusca CasB crystal structure reveals conserved positive surface charges, which we show are important for its nucleic acid binding function. EM docking reveals that CasB dimerization aligns individual nucleic acid binding surfaces into a curved, elongated binding surface inside Type I-E Cascade, consistent with the putative functions of CasB in ds-DNA recruitment and crRNA-DNA duplex formation steps. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: TthCasB and TthCasBbind by x-ray crystallography (View interaction) TfuCasB1 and TfuCasB1bind by molecular sieving (View Interaction: 1, 2). Nucleic acid binding surface and dimer interface revealed by CRISPR-associated CasB protein structures.,Nam KH, Huang Q, Ke A FEBS Lett. 2012 Nov 16;586(22):3956-61. doi: 10.1016/j.febslet.2012.09.041. Epub , 2012 Oct 16. PMID:23079036 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also CRISPR type I-E (Cascade) Endonuclease 3D structures

Proteopedia Page Contributors and Editors (what is this?)

OCA