4hdd

Domain swapping in the cytoplasmic domain of the Escherichia coli rhomboid protease

spinqualitylabelsall models 4hdd, resolution 1.35Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4hdd is a 1 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.35Å Ligands: ACT, MSE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function GLPG_ECOLI Rhomboid-type serine protease that catalyzes intramembrane proteolysis. Publication Abstract from PubMed Rhomboids are membrane-embedded serine proteases that cleave membrane protein substrates. Escherichia coli rhomboid GlpG (ecGlpG) consists of an N-terminal cytoplasmic domain and a membrane domain containing the active site. We determined the crystal structure of the soluble cytoplasmic domain of ecGlpG at 1.35A resolution and examined whether this domain affected the catalytic activity of the enzyme. The structure revealed that the ecGlpG cytoplasmic domain exists as a dimer with extensive domain swapping between the two monomers. Domain-swapped dimers can be isolated from the full-length protein, suggesting that this is a physiologically relevant structure. An extensive steady-state kinetic analysis of the full-length ecGlpG and its membrane domain using soluble and transmembrane model protein substrates resulted in an unexpected conclusion: removal of the cytoplasmic domain does not alter the catalytic parameters for detergent-solubilized rhomboid for both substrates. Domain swapping in the cytoplasmic domain of the Escherichia coli rhomboid protease.,Lazareno-Saez C, Arutyunova E, Coquelle N, Lemieux MJ J Mol Biol. 2013 Apr 12;425(7):1127-42. doi: 10.1016/j.jmb.2013.01.019. Epub 2013, Jan 23. PMID:23353827 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Rhomboid protease

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