4hyj

Crystal structure of Exiguobacterium sibiricum rhodopsin

spinqualitylabelsall models 4hyj, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4hyj is a 2 chain structure with sequence from Exiguobacterium sibiricum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.3Å Ligands: LFA, RET Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B1YFV8_EXIS2 Publication Abstract from PubMed Light-driven proton pumps are present in many organisms. Here, we present a high-resolution structure of a proteorhodopsin from a permafrost bacterium, Exiguobacterium sibiricum rhodopsin (ESR). Contrary to the proton pumps of known structure, ESR possesses three unique features. First, ESR's proton donor is a lysine side chain that is situated very close to the bulk solvent. Second, the alpha-helical structure in the middle of the helix F is replaced by 310- and pi-helix-like elements that are stabilized by the Trp-154 and Asn-224 side chains. This feature is characteristic for the proteorhodopsin family of proteins. Third, the proton release region is connected to the bulk solvent by a chain of water molecules already in the ground state. Despite these peculiarities, the positions of water molecule and amino acid side chains in the immediate Schiff base vicinity are very well conserved. These features make ESR a very unusual proton pump. The presented structure sheds light on the large family of proteorhodopsins, for which structural information was not available previously. Structural insights into the proton pumping by unusual proteorhodopsin from nonmarine bacteria.,Gushchin I, Chervakov P, Kuzmichev P, Popov AN, Round E, Borshchevskiy V, Ishchenko A, Petrovskaya L, Chupin V, Dolgikh DA, Arseniev AA, Kirpichnikov M, Gordeliy V Proc Natl Acad Sci U S A. 2013 Jul 30;110(31):12631-6. doi:, 10.1073/pnas.1221629110. Epub 2013 Jul 19. PMID:23872846 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Rhodopsin 3D structures

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