4hz8

Crystal structure of BglB with natural substrate

spinqualitylabelsall models 4hz8, resolution 1.14Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4hz8 is a 1 chain structure with sequence from Uncultured bacterium. This structure supersedes the now removed PDB entry 3fj0. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.14Å Ligands: BGC Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q0GMU3_9BACT Publication Abstract from PubMed Beta-glucosidase enzymes (EC 3.2.1-3.2.3) hydrolyze sugars and are implicated in a wide spectrum of biological processes. Recently, we reported that beta-glucosidase has varied kinetic parameters for the natural and synthetic substrates [K.H Nam, S.J. Kim, M.Y. Kim, J.H. Kim, T.S. Yeo, C.M. Lee, H.K Jun, K.Y. Hwang. Crystal structure of engineered beta-glucosidase from a soil metagenome, Proteins 73 (2008) 788-793]. However, an understanding of the kinetic values of beta-glucosidase has not yet enabled the elucidation of its molecular function. Here, we report the X-ray crystal structure of beta-glucosidase with a glucose and cellobiose fragment from uncultured soil metagenome. From the various crystals, we obtained the pre-reaction (native), intermediate (disaccharide cleavage) and post-reaction (glucose binding) states of the active site pocket. These structures provide snapshots of the catalytic processing of beta-glucosidase. In addition, the intermediate state of the crystal structure provides insight into the substrate specificity of beta-glucosidase. These structural studies will facilitate elucidation of the architectural mechanism responsible for the substrate recognition of beta-glucosidase. Structural insights into the substrate recognition properties of beta-glucosidase.,Nam KH, Sung MW, Hwang KY Biochem Biophys Res Commun. 2010 Jan 1;391(1):1131-5. doi:, 10.1016/j.bbrc.2009.12.038. Epub 2009 Dec 11. PMID:20005197 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Beta-glucosidase 3D structures

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