| Structural highlights
Function
CHS5_YEAST Component of the CHS5/6 complex which mediates export of specific cargo proteins, including chitin synthase CHS3. Also involved in targeting FUS1 to sites of polarized growth.[1] [2] [3] [4] [5] [6] [7] [8]
Publication Abstract from PubMed
Exomer is a cargo adaptor that mediates the sorting of specific plasma membrane proteins into vesicles at the trans-Golgi network. Cargo adaptors must bind to multiple partners, including their cargo, regulatory proteins, and the membrane surface. During biogenesis of a vesicle, the membrane makes a transition from a relatively flat surface to one of high curvature, requiring cargo adaptors to somehow maintain protein-protein and protein-membrane interactions on a changing membrane environment. Here, we present the crystal structure of a tetrameric Chs5/Bch1 exomer complex and use small-angle X-ray scattering to demonstrate its flexibility in solution. The structural data suggest that the complex flexes primarily around the dimeric N-terminal domain of the Chs5 subunits, which adopts a noncanonical beta sandwich fold. We propose that this flexible hinge domain enables exomer to maintain interactions in the context of a dynamic membrane environment.
The Exomer Cargo Adaptor Features a Flexible Hinge Domain.,Richardson BC, Fromme JC Structure. 2013 Feb 5. pii: S0969-2126(13)00006-3. doi:, 10.1016/j.str.2013.01.003. PMID:23395181[9]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Santos B, Duran A, Valdivieso MH. CHS5, a gene involved in chitin synthesis and mating in Saccharomyces cerevisiae. Mol Cell Biol. 1997 May;17(5):2485-96. PMID:9111317
- ↑ Choi WJ, Sburlati A, Cabib E. Chitin synthase 3 from yeast has zymogenic properties that depend on both the CAL1 and the CAL3 genes. Proc Natl Acad Sci U S A. 1994 May 24;91(11):4727-30. PMID:8197125
- ↑ Santos B, Snyder M. Specific protein targeting during cell differentiation: polarized localization of Fus1p during mating depends on Chs5p in Saccharomyces cerevisiae. Eukaryot Cell. 2003 Aug;2(4):821-5. PMID:12912901
- ↑ Lesage G, Shapiro J, Specht CA, Sdicu AM, Menard P, Hussein S, Tong AH, Boone C, Bussey H. An interactional network of genes involved in chitin synthesis in Saccharomyces cerevisiae. BMC Genet. 2005 Feb 16;6:8. PMID:15715908 doi:10.1186/1471-2156-6-8
- ↑ Trautwein M, Schindler C, Gauss R, Dengjel J, Hartmann E, Spang A. Arf1p, Chs5p and the ChAPs are required for export of specialized cargo from the Golgi. EMBO J. 2006 Mar 8;25(5):943-54. Epub 2006 Feb 23. PMID:16498409 doi:7601007
- ↑ Lam KK, Davey M, Sun B, Roth AF, Davis NG, Conibear E. Palmitoylation by the DHHC protein Pfa4 regulates the ER exit of Chs3. J Cell Biol. 2006 Jul 3;174(1):19-25. PMID:16818716 doi:jcb.200602049
- ↑ Wang CW, Hamamoto S, Orci L, Schekman R. Exomer: A coat complex for transport of select membrane proteins from the trans-Golgi network to the plasma membrane in yeast. J Cell Biol. 2006 Sep 25;174(7):973-83. PMID:17000877 doi:10.1083/jcb.200605106
- ↑ Sanchatjate S, Schekman R. Chs5/6 complex: a multiprotein complex that interacts with and conveys chitin synthase III from the trans-Golgi network to the cell surface. Mol Biol Cell. 2006 Oct;17(10):4157-66. Epub 2006 Jul 19. PMID:16855022 doi:10.1091/mbc.E06-03-0210
- ↑ Richardson BC, Fromme JC. The Exomer Cargo Adaptor Features a Flexible Hinge Domain. Structure. 2013 Feb 5. pii: S0969-2126(13)00006-3. doi:, 10.1016/j.str.2013.01.003. PMID:23395181 doi:https://dx.doi.org/10.1016/j.str.2013.01.003
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