4jbu

1.65A structure of the T3SS tip protein LcrV (G28-D322, C273S) from Yersinia pestis

spinqualitylabelsall models 4jbu, resolution 1.65Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4jbu is a 1 chain structure with sequence from Yersinia pestis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.65Å Ligands: PG4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function LCRV_YERPE Possibly involved in calcium regulation of YOP expression, which includes the export process. Publication Abstract from PubMed The human pathogen Yersinia pestis requires the assembly of the type III secretion system (T3SS) for virulence. The structural component of the T3SS contains an external needle and a tip complex, which is formed by LcrV in Y. pestis. The structure of an LcrV triple mutant (K40A/D41A/K42A) in a C273S background has previously been reported to 2.2 A resolution. Here, the crystal structure of LcrV without the triple mutation in a C273S background is reported at a higher resolution of 1.65 A. Overall the two structures are similar, but there are also notable differences, particularly near the site of the triple mutation. The refined structure revealed a slight shift in the backbone positions of residues Gly28-Asn43 and displayed electron density in the loop region consisting of residues Ile46-Val63, which was disordered in the original structure. In addition, the helical turn region spanning residues Tyr77-Gln95 adopts a different orientation. Structure of the Yersinia pestis tip protein LcrV refined to 1.65 A resolution.,Chaudhury S, Battaile KP, Lovell S, Plano GV, De Guzman RN Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 May;69(Pt 5):477-81. doi: , 10.1107/S1744309113008579. Epub 2013 Apr 27. PMID:23695558 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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