4kpi | pdb_00004kpi

Rotational order-disorder structure of reversibly photoswitchable red fluorescent protein rsTagRFP

spinqualitylabelsall models 4kpi, resolution 1.58Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4kpi is a 1 chain structure with sequence from Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.58Å Ligands: NRQ Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function D0VX33_ENTQU Publication Abstract from PubMed The rotational order-disorder (OD) structure of the reversibly photoswitchable fluorescent protein rsTagRFP is discussed in detail. The structure is composed of tetramers of 222 symmetry incorporated into the lattice in two different orientations rotated 90 degrees with respect to each other around the crystal c axis and with tetramer axes coinciding with the crystallographic twofold axes. The random distribution of alternatively oriented tetramers in the crystal creates the rotational OD structure with statistically averaged I422 symmetry. Despite order-disorder pathology, the structure of rsTagRFP has electron-density maps of good quality for both non-overlapping and overlapping parts of the model. The crystal contacts, crystal internal architecture and a possible mechanism of rotational OD crystal formation are discussed. The rotational order-disorder structure of the reversibly photoswitchable red fluorescent protein rsTagRFP.,Pletnev S, Subach FV, Verkhusha VV, Dauter Z Acta Crystallogr D Biol Crystallogr. 2014 Jan;70(Pt 1):31-9. doi:, 10.1107/S1399004713024644. Epub 2013 Dec 24. PMID:24419376 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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