4kt3

Structure of a type VI secretion system effector-immunity complex from Pseudomonas protegens

spinqualitylabelsall models 4kt3, resolution 1.44Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4kt3 is a 2 chain structure with sequence from Pseudomonas protegens Pf-5. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.4362Å Ligands: MSE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q4KC90_PSEF5 Publication Abstract from PubMed Bacteria employ type VI secretion systems (T6SSs) to facilitate antagonistic interactions towards prokaryotic and eukaryotic cells. Despite the widespread identification of T6SSs among Gram-negative bacteria, the number of experimentally validated substrate effector proteins mediating these interactions remains small. Here, employing an informatics approach, we define novel families of T6S peptidoglycan glycoside hydrolase effectors. Consistent with the known intercellular selfintoxication exhibited by the T6S pathway, we observe that each effector gene is located adjacent to a hypothetical open reading frame encoding a putative periplasmically-localized immunity determinant. To validate our sequence-based approach, we functionally investigate a representative family member from the soil-dwelling bacterium Pseudomonas protegens. We demonstrate that this protein is secreted in a T6SS-dependent manner and that it confers a fitness advantage in growth competition assays with Pseudomonas putida. In addition, we determined the 1.4 A X-ray crystal structure of this effector in complex with its cognate immunity protein. The structure reveals the effector shares highest overall structural similarity to a glycoside hydrolase family associated with peptidoglycan Nacetylglucosaminidase activity - suggesting that T6S peptidoglycan glycoside hydrolase effector families may comprise significant enzymatic diversity. Our structural analyses also demonstrate that self-intoxication is prevented by direct occlusion of the active site in a manner that would prevent binding of the murein substrate. This work significantly expands our current understanding of T6S effector diversity. Identification, structure and function of a novel type VI secretion peptidoglycan glycoside hydrolase effector-immunity pair.,Whitney JC, Chou S, Russell AB, Biboy J, Gardiner TE, Ferrin MA, Brittnacher M, Vollmer W, Mougous JD J Biol Chem. 2013 Jul 22. PMID:23878199 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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