4l0k

Crystal structure of a type II restriction endonuclease

spinqualitylabelsall models 4l0k, resolution 2.33Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4l0k is a 4 chain structure with sequence from Deinococcus radiophilus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.328Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A067XG67_9DEIO Publication Abstract from PubMed DraIII is a type IIP restriction endonucleases (REases) that recognizes and creates a double strand break within the gapped palindromic sequence CAC upward arrowNNN downward arrowGTG of double-stranded DNA ( upward arrow indicates nicking on the bottom strand; downward arrow indicates nicking on the top strand). However, wild type DraIII shows significant star activity. In this study, it was found that the prominent star site is CAT upward arrowGTT downward arrowGTG, consisting of a star 5' half (CAT) and a canonical 3' half (GTG). DraIII nicks the 3' canonical half site at a faster rate than the 5' star half site, in contrast to the similar rate with the canonical full site. The crystal structure of the DraIII protein was solved. It indicated, as supported by mutagenesis, that DraIII possesses a betabetaalpha-metal HNH active site. The structure revealed extensive intra-molecular interactions between the N-terminal domain and the C-terminal domain containing the HNH active site. Disruptions of these interactions through site-directed mutagenesis drastically increased cleavage fidelity. The understanding of fidelity mechanisms will enable generation of high fidelity REases. Elimination of inter-domain interactions increases the cleavage fidelity of the restriction endonuclease DraIII.,Zhuo W, Lai X, Zhang L, Chan SH, Li F, Zhu Z, Yang M, Sun D Protein Cell. 2014 May;5(5):357-68. doi: 10.1007/s13238-014-0038-z. Epub 2014 Apr, 15. PMID:24733184 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Endonuclease 3D structures

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