4lcv
Crystal Structure of DOC2B C2A domain
Structural highlights
FunctionDOC2B_RAT Calcium sensor which positively regulates SNARE-dependent fusion of vesicles with membranes. Binds phospholipids in a calcium-dependent manner and may act at the priming stage of fusion by modifying membrane curvature to stimulate fusion. Involved in calcium-triggered exocytosis in chromaffin cells and calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Involved both in glucose-stimulated insulin secretion in pancreatic cells and insulin-dependent GLUT4 transport to the plasma membrane in adipocytes.[1] [2] Publication Abstract from PubMedDOC2B (double-C2 domain) protein is thought to be a high-affinity Ca2+ sensor for spontaneous and asynchronous neurotransmitter release. To elucidate the molecular features underlying its physiological role, we determined the crystal structures of its isolated C2A and C2B domains and examined their Ca2+-binding properties. We further characterized the solution structure of the tandem domains (C2AB) using small-angle X-ray scattering. In parallel, we tested structure-function correlates with live cell imaging tools. We found that, despite striking structural similarity, C2B binds Ca2+ with considerably higher affinity than C2A. The C2AB solution structure is best modeled as two domains with a highly flexible orientation and no difference in the presence or absence of Ca2+. In addition, kinetic studies of C2AB demonstrate that, in the presence of unilamellar vesicles, Ca2+ binding is stabilized, as reflected by the ~10-fold slower rate of Ca2+ dissociation than in the absence of vesicles. In cells, isolated C2B translocates to the plasma membrane (PM) with an EC50 of 400nM while the C2A does not translocate at submicromolar Ca2+ concentrations, supporting the biochemical observations. Nevertheless, C2AB translocates to the PM with an ~2-fold lower EC50 and to a greater extent than C2B. Our results, together with previous studies, reveal that the C2B is the primary Ca2+ sensing unit in DOC2B, whereas C2A enhances the interaction of C2AB with the PM. The C2B Domain Is the Primary Ca Sensor in DOC2B: A Structural and Functional Analysis.,Giladi M, Michaely L, Almagor L, Bar-On D, Buki T, Ashery U, Khananshvili D, Hirsch JA J Mol Biol. 2013 Aug 28. pii: S0022-2836(13)00537-8. doi:, 10.1016/j.jmb.2013.08.017. PMID:23994332[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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