4lup

Crystal structure of the complex formed by region of E. coli sigmaE bound to its -10 element non template strand

spinqualitylabelsall models 4lup, resolution 1.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4lup is a 4 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.2Å Ligands: EDO Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q0P6M2_ECOLX Publication Abstract from PubMed Bacterial transcription is controlled by sigma factors, the RNA polymerase subunits that act as initiation factors. Although a single housekeeping sigma factor enables transcription from thousands of promoters, environmentally induced sigma factors redirect gene expression toward small regulons to carry out focused responses. Using structural and functional analyses, we determined the molecular basis of -10 promoter element recognition by Escherichia coli sigmaE, which revealed an unprecedented way to achieve promoter melting. Group IV sigma factors induced strand separation at the -10 element by flipping out a single nucleotide from the nontemplate-strand DNA base stack. Unambiguous selection of this critical base was driven by a dynamic protein loop, which can be substituted to modify specificity of promoter recognition. This mechanism of promoter melting explains the increased promoter-selection stringency of environmentally induced sigma factors. Structural basis for -10 promoter element melting by environmentally induced sigma factors.,Campagne S, Marsh ME, Capitani G, Vorholt JA, Allain FH Nat Struct Mol Biol. 2014 Feb 16. doi: 10.1038/nsmb.2777. PMID:24531660 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Sigma factor Sigma factor 3D structures

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