4mzm

MazF from S. aureus crystal form I, P212121, 2.1 A

spinqualitylabelsall models 4mzm, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4mzm is a 4 chain structure with sequence from Staphylococcus aureus subsp. aureus N315. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.1Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function MAZF_STAAN Toxic component of a toxin-antitoxin (TA) module. Ribosome-independent, sequence-specific endoribonuclease that cleaves mRNA, thus inhibiting protein synthesis and inducing bacterial stasis. Cleavages occur preferentially in a U-rich region with a consensus sequence of 5'-[ACG]UU[ACG]-3' in single-stranded RNA (By similarity). Publication Abstract from PubMed The Staphylococcus aureus genome contains three toxin-antitoxin modules, including one mazEF module, SamazEF. Using an on-column separation protocol we are able to obtain large amounts of wild-type SaMazF toxin. The protein is well-folded and highly resistant against thermal unfolding but aggregates at elevated temperatures. Crystallographic and nuclear magnetic resonance (NMR) solution studies show a well-defined dimer. Differences in structure and dynamics between the X-ray and NMR structural ensembles are found in three loop regions, two of which undergo motions that are of functional relevance. The same segments also show functionally relevant dynamics in the distantly related CcdB family despite divergence of function. NMR chemical shift mapping and analysis of residue conservation in the MazF family suggests a conserved mode for the inhibition of MazF by MazE. Structural and biophysical characterization of Staphylococcus aureus SaMazF shows conservation of functional dynamics.,Zorzini V, Buts L, Sleutel M, Garcia-Pino A, Talavera A, Haesaerts S, Greve HD, Cheung A, van Nuland NA, Loris R Nucleic Acids Res. 2014 Jun 1;42(10):6709-25. doi: 10.1093/nar/gku266. Epub 2014 , Apr 19. PMID:24748664 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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