4oij

X-ray crystal structure of racemic non-glycosylated chemokine Ser-CCL1

spinqualitylabelsall models 4oij, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4oij is a 4 chain structure with sequence from Homo sapiens and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2Å Ligands: DAL, DAR, DAS, DCY, DGL, DGN, DHI, DIL, DLE, DLY, DPN, DPR, DSG, DSN, DTH, DTR, DTY, DVA, MED, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CCL1_HUMAN Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8. Publication Abstract from PubMed Our goal was to obtain the X-ray crystal structure of the glycosylated chemokine Ser-CCL1. Glycoproteins can be hard to crystallize because of the heterogeneity of the oligosaccharide (glycan) moiety. We used glycosylated Ser-CCL1 that had been prepared by total chemical synthesis as a homogeneous compound containing an N-linked asialo biantennary nonasaccharide glycan moiety of defined covalent structure. Facile crystal formation occurred from a quasi-racemic mixture consisting of glycosylated L-protein and non-glycosylated-D-protein, while no crystals were obtained from the glycosylated L-protein alone. The structure was solved at a resolution of 2.6-2.1 A. However, the glycan moiety was disordered: only the N-linked GlcNAc sugar was well-defined in the electron density map. A racemic mixture of the protein enantiomers L-Ser-CCL1 and D-Ser-CCL1 was also crystallized, and the structure of the true racemate was solved at a resolution of 2.7-2.15 A. Superimposition of the structures of the protein moieties of L-Ser-CCL1 and glycosylated-L-Ser-CCL1 revealed there was no significant alteration of the protein structure by N-glycosylation. (Quasi-)Racemic X-ray Structures of Glycosylated and Non-Glycosylated Forms of the Chemokine Ser-CCL1 Prepared by Total Chemical Synthesis.,Okamoto R, Mandal K, Sawaya MR, Kajihara Y, Yeates TO, Kent SB Angew Chem Int Ed Engl. 2014 Apr 1. doi: 10.1002/anie.201400679. PMID:24692304 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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