4omy

Crystal Structure of SeMet NolR from Sinorhizobium fredii in complex with oligo AT DNA

spinqualitylabelsall models 4omy, resolution 3.06Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4omy is a 8 chain structure with sequence from Sinorhizobium fredii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.062Å Ligands: MSE Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q83TD2_RHIFR Publication Abstract from PubMed The symbiosis between rhizobial microbes and host plants involves the coordinated expression of multiple genes, which leads to nodule formation and nitrogen fixation. As part of the transcriptional machinery for nodulation and symbiosis across a range of Rhizobium, NolR serves as a global regulatory protein. Here, we present the X-ray crystal structures of NolR in the unliganded form and complexed with two different 22-base pair (bp) double-stranded operator sequences (oligos AT and AA). Structural and biochemical analysis of NolR reveals protein-DNA interactions with an asymmetric operator site and defines a mechanism for conformational switching of a key residue (Gln56) to accommodate variation in target DNA sequences from diverse rhizobial genes for nodulation and symbiosis. This conformational switching alters the energetic contributions to DNA binding without changes in affinity for the target sequence. Two possible models for the role of NolR in the regulation of different nodulation and symbiosis genes are proposed. To our knowledge, these studies provide the first structural insight on the regulation of genes involved in the agriculturally and ecologically important symbiosis of microbes and plants that leads to nodule formation and nitrogen fixation. Structural basis for regulation of rhizobial nodulation and symbiosis gene expression by the regulatory protein NolR.,Lee SG, Krishnan HB, Jez JM Proc Natl Acad Sci U S A. 2014 Apr 29;111(17):6509-14. doi:, 10.1073/pnas.1402243111. Epub 2014 Apr 14. PMID:24733893 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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